The Phospho-Stat5 (Y694) Polyclonal Antibody (CABP0887) is a powerful research tool designed for the detection and analysis of phosphorylated Stat5 protein. Stat5 is a key transcription factor involved in cellular signaling pathways, particularly in the regulation of immune responses and cell proliferation.This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By targeting the phosphorylated form of Stat5 at the Y694 site, this antibody enables researchers to investigate the activation status of Stat5 in different cell types and under various experimental conditions.The phosphorylation of Stat5 at Y694 plays a crucial role in signal transduction cascades that control gene expression and cellular functions.
Dysregulation of Stat5 phosphorylation has been implicated in cancer, autoimmune diseases, and other pathological conditions, making this antibody an invaluable tool for studies in immunology, oncology, and molecular biology.By providing insights into the activation of Stat5 at the molecular level, this antibody holds promise for identifying potential therapeutic targets and developing novel treatment strategies for diseases associated with dysregulated immune responses and cell growth. Explore the functional significance of phosphorylated Stat5 with the Phospho-Stat5 (Y694) Polyclonal Antibody (CABP0887) and advance your research in the field of cellular signaling and disease biology.
Product Name:
Phospho-STAT5-Y694 Rabbit Polyclonal Antibody
SKU:
CABP0887
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y694 of human STAT5 (NP_003143.2/NP_036580.2).
Sequence:
DGYV K
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:100 - 1:500 IHC-P,1:100 - 1:200
Synonyms:
MGF; STAT5; stat5a/stat5b; Phospho-STAT5-Y694
Positive Sample:
NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
cytosol, nucleoplasm
Calculated MW:
90kDa
Observed MW:
90kDa
Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Purification Method:
Affinity purification
Gene ID:
6776/6777
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT5-Y694 Rabbit pAb (CABP0887) at 1:400 dilution.NIH/3T3 cells were treated by Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
