The Phospho-Stat3 (Y705) Rabbit Monoclonal Antibody (CABP0705) is a powerful tool for researchers studying signal transduction pathways involving Stat3, a transcription factor activated by phosphorylation at tyrosine 705. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot and immunohistochemistry applications.Stat3 activation at tyrosine 705 is known to play a key role in regulating gene expression related to cell proliferation, survival, and differentiation. Dysregulation of Stat3 signaling has been implicated in various diseases including cancer, inflammation, and autoimmune disorders.
This antibody enables researchers to specifically detect and analyze phosphorylated Stat3 in a variety of cell types, providing valuable insights into the mechanisms underlying these pathological conditions.With its ability to target phospho-Stat3 at tyrosine 705, this antibody is a valuable tool for investigating the role of Stat3 signaling in disease development and progression. Its use in immunology and cancer research studies can help unravel the complexities of these processes and potentially lead to the development of novel therapeutic strategies targeting Stat3 activation.
Product Name:
Phospho-STAT3-Y705 Rabbit Monoclonal Antibody
SKU:
CABP0705
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y705 of human STAT3 (NP_644805.1).
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.
Purification Method:
Affinity purification
Gene ID:
6774
Clone Number:
ARC50831
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT3-Y705 Rabbit mAb (CABP0705) at 1:1000 dilution.NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
