The Phospho-Stat3 (S727) Rabbit Monoclonal Antibody (CABP0715) is a crucial tool for researchers studying the phosphorylation status of Stat3, a key signaling molecule involved in various cellular processes including inflammation, cell growth, and survival. This antibody, generated using rabbit monoclonal technology, specifically targets the phosphorylated form of Stat3 at the S727 residue, making it an essential tool for studies focusing on the activation and regulation of Stat3 signaling pathways.The antibody is highly specific to human samples and has been validated for use in Western blot applications, offering researchers a reliable method for detecting and analyzing phosphorylated Stat3 levels in different cell types.
By targeting the Phospho-Stat3 (S727) form, researchers can gain insight into the activation status of Stat3 and its role in diseases such as cancer, autoimmune disorders, and inflammatory conditions.With its ability to selectively detect the phosphorylated form of Stat3, the Phospho-Stat3 (S727) Rabbit Monoclonal Antibody (CABP0715) is a valuable tool for researchers exploring the intricate mechanisms of signaling pathways and potential therapeutic targets in various disease contexts. Its high specificity and reliability make it an essential reagent for studies in immunology, cancer research, and other fields where Stat3 signaling plays a pivotal role.
Product Name:
Phospho-STAT3-S727 Rabbit Monoclonal Antibody
SKU:
CABP0715
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S727 of human Phospho-Phospho-STAT3-S727 (S727) (P40763).
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.
Purification Method:
Affinity purification
Gene ID:
6774
Clone Number:
ARC0150
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-STAT3-S727 Rabbit mAb (CABP0715) at 1:1000 dilution.NIH/3T3 and PC-12 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 120s.
