The Phospho-Stat3 (S727) Polyclonal Antibody (CABP0474) is specifically designed for research involving the phosphorylated form of Stat3 at serine 727. Stat3 is a transcription factor that plays a key role in various cellular processes, including cell growth, differentiation, and survival. The phosphorylation of Stat3 at serine 727 is known to enhance its transcriptional activity and is important for its role in regulating gene expression.This antibody, raised in rabbits, is highly reactive with human samples and has been validated for Western blot applications. It specifically binds to the phosphorylated form of Stat3 at serine 727, allowing for the detection and analysis of this important signaling molecule in various cell types.
This makes it an ideal tool for studies in signal transduction, cancer biology, and inflammation research.Understanding the phosphorylation status of Stat3 at serine 727 is crucial for deciphering its role in different cellular pathways and disease states. The Phospho-Stat3 (S727) Polyclonal Antibody provides researchers with a reliable tool to investigate the functional consequences of this post-translational modification and its implications in various physiological and pathological conditions.
Product Name:
Phospho-STAT3-S727 Rabbit Polyclonal Antibody
SKU:
CABP0474
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S727 of human Phospho-STAT3-S727 (NP_644805.1).
Sequence:
PMSP R
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
APRF; HIES; ADMIO; ADMIO1; Phospho-STAT3-S727
Positive Sample:
HeLa,HepG2,C6
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
88kDa
Observed MW:
86kDa
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.
Purification Method:
Affinity purification
Gene ID:
6774
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-STAT3-S727 Rabbit pAb (CABP0474) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
