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Phospho-STAT1-Y701 Rabbit Monoclonal Antibody (CABP0054)

SKU:
CABP0054
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Immunology
$503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-STAT1-Y701 Rabbit Monoclonal Antibody
SKU:CABP0054
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around Y701 of human STAT1 (P42224).
Sequence:TGYI K
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91; Phospho-STAT1-Y701
Positive Sample:HeLa+IFN-α,NIH/3T3+IFN-α,PC-12+IFN-α
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:87kDa
Observed MW:91kDa

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.

Purification Method:Affinity purification
Gene ID:6772
Clone Number:ARC0049
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-STAT1-Y701 Rabbit mAb (CABP0054) at 1:1000 dilution.HeLa,NIH/3T3 and PC-12 cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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