The Phospho-STAT1 (Y701) Rabbit Monoclonal Antibody (CABP0054) is a powerful tool for research into intracellular signaling pathways involving STAT1, a transcription factor that plays a key role in mediating responses to cytokines and growth factors. This antibody, produced in rabbits, specifically recognizes phosphorylated STAT1 at tyrosine 701 and has been validated for use in various experimental applications, including Western blotting and immunofluorescence.Phosphorylation of STAT1 at tyrosine 701 is a crucial step in the activation of this transcription factor, leading to the regulation of genes involved in immune response, cell proliferation, and apoptosis. Research into the phosphorylation status of STAT1 can provide valuable insights into the mechanisms underlying various diseases, including cancer, immune disorders, and infectious diseases.
By targeting the phosphorylated form of STAT1, researchers can gain a better understanding of how this signaling pathway is dysregulated in different pathological conditions.Overall, the Phospho-STAT1 (Y701) Rabbit Monoclonal Antibody (CABP0054) is a reliable tool for studying the activation and function of STAT1 in a variety of research contexts, making it a valuable asset for scientists interested in unraveling the complexities of intracellular signaling pathways and developing targeted therapies for disease intervention.
Product Name:
Phospho-STAT1-Y701 Rabbit Monoclonal Antibody
SKU:
CABP0054
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y701 of human STAT1 (P42224).
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.
Purification Method:
Affinity purification
Gene ID:
6772
Clone Number:
ARC0049
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-STAT1-Y701 Rabbit mAb (CABP0054) at 1:1000 dilution.HeLa,NIH/3T3 and PC-12 cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
