The Phospho-Stat1 (Y701) Polyclonal Antibody (CABP0135) is a valuable tool for researchers studying signal transduction pathways involved in immune responses and inflammation. This antibody, generated in rabbits, specifically recognizes phosphorylated Stat1 at tyrosine 701, a key residue important for Stat1 activation.Stat1 is a transcription factor that plays a crucial role in mediating cellular responses to cytokines and growth factors, particularly in the context of antiviral and inflammatory signaling pathways. Phosphorylation of Stat1 at tyrosine 701 is essential for its dimerization, nuclear translocation, and subsequent activation of target genes involved in immune regulation.The Phospho-Stat1 (Y701) Polyclonal Antibody (CABP0135) is validated for use in Western blot applications, allowing for the detection and analysis of phosphorylated Stat1 in various cell types and tissues.
Its high specificity and sensitivity make it an ideal tool for investigating the role of Stat1 phosphorylation in disease states such as cancer, autoimmune disorders, and infectious diseases.By providing researchers with a reliable means to study Stat1 activation, this antibody enables a deeper understanding of the complex mechanisms underlying immune responses and inflammatory processes. Its utility in both basic research and drug development makes it a valuable asset for scientists seeking to unravel the intricacies of signal transduction pathways in health and disease.
Product Name:
Phospho-STAT1-Y701 Rabbit Polyclonal Antibody
SKU:
CABP0135
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y701 of human STAT1 (NP_009330.1).
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.
Purification Method:
Affinity purification
Gene ID:
6772
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Hela cells, using Phospho-STAT1-Y701 (CABP0135) at 1:630 dilution.HeLa cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
