The Phospho-STAT1 (S727) Rabbit Monoclonal Antibody (CABP1000) is a cutting-edge tool for researchers studying signal transducer and activator of transcription 1 (STAT1) phosphorylated at serine 727. This antibody, developed by Assay Genie, is specifically designed for use in various applications such as Western blot and immunohistochemistry to detect and analyze the phosphorylation status of STAT1 at serine 727 in human samples.STAT1 is a key transcription factor involved in the regulation of immune response, cell growth, and differentiation. Phosphorylation of STAT1 at serine 727 is known to enhance its transcriptional activity and influence its function in various cellular processes.
Therefore, understanding the phosphorylation status of STAT1 at this specific site is crucial for researchers investigating immune signaling pathways, viral infections, and cancer biology.This high-quality antibody, raised in rabbits, offers exceptional sensitivity and specificity for detecting phosphorylated STAT1 at serine 727, making it a valuable tool for exploring the role of this post-translational modification in cellular signaling pathways. With its reliable performance and robust validation, the Phospho-STAT1 (S727) Rabbit Monoclonal Antibody (CABP1000) from Assay Genie is an essential reagent for advancing research in immunology, oncology, and molecular biology.
Product Name:
Phospho-STAT1-S727 Rabbit Monoclonal Antibody
SKU:
CABP1000
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S727 of human STAT1 (P42224).
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.
Purification Method:
Affinity purification
Gene ID:
6772
Clone Number:
ARC1544
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-STAT1-S727 Rabbit mAb (CABP1000) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
