The Phospho-STAT1 (S727) Polyclonal Antibody (CABP0453) is a valuable tool for researchers studying signal transducer and activator of transcription 1 (STAT1), a key protein involved in the regulation of immune responses. This antibody, produced in rabbits, specifically targets the phosphorylated form of STAT1 at serine 727 and is highly sensitive for detecting this post-translational modification in human samples.Phosphorylation of STAT1 at serine 727 is known to play a critical role in modulating the transcriptional activity of STAT1, influencing the expression of genes involved in immune signaling pathways. The ability to detect and quantify this phosphorylation event is essential for understanding how STAT1 function is regulated in various cellular contexts, particularly in the fields of immunology and cancer research.
By using the Phospho-STAT1 (S727) Polyclonal Antibody, researchers can gain insights into the activation status of STAT1 and its downstream signaling pathways in response to different stimuli. This antibody is validated for use in Western blot applications, enabling precise and reliable detection of phospho-STAT1 (S727) levels in cell lysates and tissue samples. Its high reactivity and specificity make it a valuable tool for investigating the role of STAT1 phosphorylation in immune responses and disease development.
Product Name:
Phospho-STAT1-S727 Rabbit Polyclonal Antibody
SKU:
CABP0453
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S727 of human STAT1 (NP_009330.1).
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.
Purification Method:
Affinity purification
Gene ID:
6772
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293 cells, using Phospho-STAT1-S727 Rabbit pAb (CABP0453).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
