The Phospho-Stat1 (S727) Polyclonal Antibody (CABP0109) is a valuable tool for researchers studying the phosphorylation of Stat1 at serine 727. This post-translational modification plays a crucial role in the activation of Stat1, a key transcription factor involved in the regulation of immune responses, cell growth, and apoptosis.Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the phosphorylated form of Stat1, researchers can accurately detect and analyze the activity of this transcription factor in various cell types, providing valuable insights into signaling pathways involved in immune regulation, cancer progression, and inflammatory responses.The phosphorylation of Stat1 at serine 727 is known to enhance its transcriptional activity and promote the expression of genes involved in antiviral defense, cell differentiation, and immune regulation.
Therefore, understanding the role of this post-translational modification is essential for unraveling the complex mechanisms underlying immune responses and for developing targeted therapies for diseases such as cancer, viral infections, and autoimmune disorders.Overall, the Phospho-Stat1 (S727) Polyclonal Antibody is a powerful tool for researchers interested in studying the phosphorylation-dependent functions of Stat1 and its role in immune signaling pathways, making it a valuable addition to immunology, oncology, and molecular biology research.
Product Name:
Phospho-STAT1-S727 Rabbit Polyclonal Antibody
SKU:
CABP0109
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S727 of human STAT1 (NP_009330.1).
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.
Purification Method:
Affinity purification
Gene ID:
6772
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-STAT1-S727 Rabbit pAb (CABP0109) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
