The Phospho-SRF (S103) Polyclonal Antibody (CABP0829) is a valuable tool for researchers studying the phosphorylation of Serum Response Factor (SRF) at Ser103. SRF is a transcription factor that plays a crucial role in regulating gene expression in response to various stimuli, including growth factors and stress signals. Phosphorylation of SRF at Ser103 has been shown to modulate its transcriptional activity, making it an important site for studying SRF function.This antibody, raised in rabbits, is highly specific for detecting phospho-SRF (S103) in human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of SRF, researchers can investigate the signaling pathways and cellular processes that regulate SRF activity.
This antibody is an essential tool for studies in cell signaling, gene regulation, and development, providing valuable insights into the mechanisms controlling gene expression in various physiological and pathological conditions.Overall, the Phospho-SRF (S103) Polyclonal Antibody is a reliable and specific reagent for researchers interested in exploring the role of phosphorylation in SRF function and its implications for understanding cellular responses to extracellular stimuli. Its versatility and high specificity make it a valuable asset for studies in molecular biology, signal transduction, and disease mechanisms.
Product Name:
Phospho-SRF-S103 Rabbit Polyclonal Antibody
SKU:
CABP0829
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S103 of human SRF (NP_003122.1).
Sequence:
SLSE M
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:100
Synonyms:
MCM1; Phospho-SRF-S103
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
52kDa
Observed MW:
70kDa
This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6722
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-SRF-S103 Rabbit pAb (CABP0829) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
