The Phospho-Src (Y529) Polyclonal Antibody (CABP0185) is a valuable tool for researchers studying the phosphorylation status of the Src protein, a key regulator of cell growth, proliferation, and survival. This antibody is raised in rabbits and specifically binds to phosphorylated Src at residue Y529, allowing for precise detection and analysis of this important post-translational modification.Phosphorylation of Src at Y529 is known to regulate its activity and downstream signaling pathways, making it a critical player in various cellular processes.
By using this antibody in Western blot applications, researchers can gain insights into the functional role of phosphorylated Src in different cell types and experimental conditions.Overall, the Phospho-Src (Y529) Polyclonal Antibody is an essential tool for investigating the complex regulatory network involving Src and its phosphorylation status. Its high reactivity with human samples and validation for Western blotting make it a reliable choice for researchers in the fields of cell biology, cancer research, and signal transduction studies.
Product Name:
Phospho-Src-Y529 Rabbit Polyclonal Antibody
SKU:
CABP0185
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y529 of human Src (NP_005408.1).
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6714
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of A-431 cells, using Phospho-Src-Y529 antibody (CABP0185) at 1:1000 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
