The Phospho-Src (Y529) Monoclonal Antibody is a valuable tool for researchers studying signaling pathways involving the phosphorylation of Src tyrosine kinase at residue Y529. Phosphorylation of this site is known to regulate the activity of Src, a key player in cell proliferation, differentiation, and survival.This antibody, generated using high-quality hybridoma technology, specifically targets the phosphorylated Y529 residue of Src tyrosine kinase, allowing for precise detection and analysis in various experimental settings. It is validated for use in Western blotting applications and has been shown to exhibit high specificity and sensitivity when used with human samples.
The phosphorylation status of Src at Y529 is associated with various cellular processes, including cell migration, adhesion, and invasion, highlighting the importance of studying this modification in the context of cancer, cell signaling, and other disease-related research areas. By using the Phospho-Src (Y529) Monoclonal Antibody, researchers can gain valuable insights into the regulatory mechanisms governing Src activity and its implications in disease progression.
Product Name:
Phospho-Src-Y529 Monoclonal Antibody
SKU:
CABP1377
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y529 of human Src.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6714
Clone Number:
ARC54665
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of A-431, using Phospho-Src-Y529 antibody (CABP1377) at 1:10000 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
