The Phospho-SRC Y419 Rabbit Monoclonal Antibody (CABP1027) is a powerful tool for researchers studying cellular signaling pathways involving the SRC protein. SRC is a tyrosine kinase that plays a key role in multiple cellular processes, including cell growth, proliferation, and differentiation. Phosphorylation of SRC at tyrosine 419 is a critical event that regulates its activity and downstream signaling.This antibody, raised in rabbits, is highly specific for the phosphorylated form of SRC at tyrosine 419 and has been validated for use in Western blot applications.
It enables researchers to detect and analyze the phosphorylation status of SRC in various cell types, providing insights into its role in cancer, cell migration, and proliferation.Understanding the phosphorylation of SRC at tyrosine 419 is essential for unraveling the complex network of signaling pathways in which SRC is involved. This antibody is a valuable tool for researchers investigating the role of SRC in cancer progression, metastasis, and drug resistance, as well as in other diseases and disorders where aberrant SRC signaling plays a role.
Product Name:
Phospho-Src-Y419 Rabbit Monoclonal Antibody
SKU:
CABP1027
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y419 of human SRC (P12931).
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6714
Clone Number:
ARC1572
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb (CABP1027) at 1:1000 dilution.NIH/3T3 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
