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Phospho-Src-Y419 Rabbit Monoclonal Antibody (CABP1027)

SKU:
CABP1027
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Src-Y419 Rabbit Monoclonal Antibody
SKU:CABP1027
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around Y419 of human SRC (P12931).
Sequence:NEYT A
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:ASV; SRC1; THC6; c-SRC; p60-Src; Phospho-Src-Y419
Positive Sample:NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, cytoskeleton.
Calculated MW:60kDa
Observed MW:60kDa

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Purification Method:Affinity purification
Gene ID:6714
Clone Number:ARC1572
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb (CABP1027) at 1:1000 dilution.NIH/3T3 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

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