The Phospho-Src (Y416) Polyclonal Antibody (CABP0452) is a valuable tool for researchers studying the activation of Src, a key protein involved in cell signaling and growth. This antibody, raised in rabbits, specifically targets phosphorylated Src at tyrosine 416 and is highly reactive with human samples. Validated for use in Western blot applications, this antibody enables the detection and analysis of activated Src in various cell types.Src is a non-receptor protein tyrosine kinase that plays a crucial role in regulating cell proliferation, survival, and differentiation. Phosphorylation of Src at tyrosine 416 is a critical event that activates its kinase activity and leads to downstream signaling pathways involved in tumorigenesis and metastasis.
Understanding the dynamics of Src activation is essential for elucidating its role in cancer progression and identifying potential therapeutic targets for cancer treatment.Overall, the Phospho-Src (Y416) Polyclonal Antibody is a valuable tool for researchers investigating the mechanisms of Src activation and its implications in cancer biology. Its high specificity and reactivity make it an ideal choice for studies in oncology, cell signaling, and drug development targeting Src-dependent pathways.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2534/3055/3932/4067/6714/7525
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb (CABP0452) at 1:1000 dilution.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
