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Phospho-SRC Family-Y416 Rabbit Polyclonal Antibody (CABP0452)

SKU:
CABP0452
Product Type:
Antibody
Applications:
WB
IHC
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€419
Frequently bought together:

Description

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Product Name:Phospho-SRC Family-Y416 Rabbit Polyclonal Antibody
SKU:CABP0452
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around Y416 of human SRC Family (NP_005408.1).
Sequence:NEYT A
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:ASV; SRC1; THC6; c-SRC; p60-Src; SRC; Phospho-SRC Family-Y416
Positive Sample:NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, cytoskeleton.
Calculated MW:60kDa
Observed MW:60kDa

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Purification Method:Affinity purification
Gene ID:2534/3055/3932/4067/6714/7525
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb (CABP0452) at 1:1000 dilution.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.

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