The Phospho-Src (S17) Polyclonal Antibody (CABP0522) is a valuable tool for researchers studying phosphorylation events in cellular signaling pathways. This antibody, generated in rabbits, specifically binds to the phosphorylated form of Src at serine 17, allowing for the detection and analysis of this key regulatory protein. It is ideal for use in Western blot applications and is highly reactive with human samples.Src is a non-receptor tyrosine kinase that plays a crucial role in cell growth, proliferation, and survival. Phosphorylation of Src at serine 17 has been implicated in various cellular processes, including cell migration, invasion, and adhesion.
Understanding the regulation of Src phosphorylation is essential for unraveling its complex functions in normal cellular physiology and pathological conditions, such as cancer.This phospho-specific antibody provides researchers with a powerful tool to study the dynamic regulation of Src signaling pathways. By targeting the phosphorylated form of Src at serine 17, researchers can gain valuable insights into the role of this protein in cancer progression, metastasis, and drug resistance. Additionally, this antibody may have broader implications in the development of targeted therapies for cancer and other diseases driven by aberrant Src signaling.
Product Name:
Phospho-Src-S17 Rabbit Polyclonal Antibody
SKU:
CABP0522
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S17 of human Phospho-Src-S17 (NP_005408.1).
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6714
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-Src-S17 antibody (CABP0522) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
