The Phospho-SQSTM1 (p62) S349 Antibody (CAB20737) is a valuable tool for researchers studying the phosphorylation of the SQSTM1 (p62) protein at serine 349. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the phosphorylated form of SQSTM1 (p62) at serine 349, this antibody allows for the detection and analysis of this critical post-translational modification in various cell types.SQSTM1 (p62) is known to play a key role in cellular processes such as selective autophagy and signal transduction, making it a crucial protein for research in fields such as cell biology and cancer biology.
Phosphorylation at serine 349 has been linked to the regulation of autophagy and protein degradation pathways, highlighting the importance of studying this modification in disease contexts.By using the Phospho-SQSTM1 (p62) S349 Antibody (CAB20737), researchers can gain insights into the functional implications of phosphorylation at serine 349 on SQSTM1 (p62) and its role in various cellular pathways. This antibody is an essential tool for studies aimed at understanding the molecular mechanisms underlying autophagy regulation and its impact on disease processes.
Product Name:
Phospho-SQSTM1/p62-S349 Polyclonal Antibody
SKU:
CAB20737
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S349 of human SQSTM1/p62 (NP_003891.1).
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Purification Method:
Affinity purification
Gene ID:
8878
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SQSTM1/p62-S349 Rabbit pAb (CAB20737) at 1:500 dilution.NIH/3T3 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
