The Phospho-Sp1 (T739) Polyclonal Antibody (CABP0231) is an essential tool for researchers studying the Sp1 transcription factor, a key regulator of gene expression. This antibody, produced in rabbits, is highly specific for the phosphorylated form of Sp1 at threonine 739, making it ideal for detecting and analyzing Sp1 activation in various experimental systems.Sp1 is a critical transcription factor that controls the expression of genes involved in cell growth, differentiation, and apoptosis. Phosphorylation of Sp1 at T739 has been linked to its activation and involvement in various cellular processes, including proliferation and migration.
Understanding the signaling pathways that regulate Sp1 phosphorylation can provide valuable insights into the molecular mechanisms underlying cancer, cardiovascular diseases, and other pathological conditions.The Phospho-Sp1 (T739) Polyclonal Antibody is validated for use in Western blotting, immunoprecipitation, and immunofluorescence, allowing for versatile applications in basic research and drug development. By targeting the phosphorylated form of Sp1, this antibody offers a unique opportunity to study the role of Sp1 in disease progression and potential therapeutic interventions.
Product Name:
Phospho-SP1-T739 Rabbit Polyclonal Antibody
SKU:
CABP0231
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding T739 of human SP1
Tested Applications:
WB
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
SP1; Phospho-SP1-T739
Positive Sample:
3T3
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
81kDa
Observed MW:
90kDa
The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6667
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 3T3 cells, using Phospho-SP1-T739 Rabbit pAb (CABP0231).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
