The SOX9 Rabbit Monoclonal Antibody (CAB19710) is a valuable tool for researchers studying the transcription factor SOX9, which plays a crucial role in the regulation of embryonic development and cell differentiation. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in a variety of applications, including Western blotting.SOX9 is known for its involvement in various biological processes, including chondrogenesis, sex determination, and stem cell maintenance. Dysregulation of SOX9 has been implicated in numerous diseases, such as skeletal disorders, campomelic dysplasia, and certain types of cancer.
Therefore, understanding the function and regulation of SOX9 is essential for uncovering its role in development and disease progression.Researchers in the fields of developmental biology, regenerative medicine, and oncology will find the SOX9 Rabbit Monoclonal Antibody (CAB19710) to be a valuable asset in their investigations. Its high specificity and sensitivity make it an ideal choice for detecting and analyzing SOX9 expression in various cell types, providing valuable insights into its biological functions and potential therapeutic applications.
Product Name:
SOX9 Rabbit Monoclonal Antibody
SKU:
CAB19710
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 169-300 of human SOX9 (P48436).
WB,1:500 - 1:2000 IHC-P,1:100 - 1:500 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CMD1; SRA1; CMPD1; SRXX2; SRXY10; SOX9
Positive Sample:
Hep G2,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
56kDa
Observed MW:
56kDa
The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
Purification Method:
Affinity purification
Gene ID:
6662
Clone Number:
ARC0190
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using SOX9 Rabbit mAb (CAB19710) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
