The Smoothened Homolog Polyclonal Antibody (CAB3274) is a valuable tool for researchers studying the Smoothened Homolog protein, a crucial component in the Hedgehog signaling pathway. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the Smoothened Homolog protein, this antibody enables accurate detection and analysis in a variety of cell types, making it a versatile option for studies in developmental biology, cancer research, and regenerative medicine.The Smoothened Homolog protein plays a key role in cellular signaling pathways that are essential for proper embryonic development and tissue maintenance. Dysregulation of the Hedgehog pathway, in which Smoothened Homolog is a key player, has been implicated in various diseases, including cancer and developmental disorders.
By understanding the function and regulation of the Smoothened Homolog protein, researchers can gain important insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.Overall, the Smoothened Homolog Polyclonal Antibody (CAB3274) provides researchers with a powerful tool for exploring the role of the Smoothened Homolog protein in cellular signaling and disease pathogenesis. Its high specificity and reactivity make it an invaluable asset for advancing our understanding of molecular mechanisms involved in development, cancer,
Product Name:
SMO Rabbit Polyclonal Antibody
SKU:
CAB3274
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 28-233 of human SMO (NP_005622.1).
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
Purification Method:
Affinity purification
Gene ID:
6608
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SMO antibody (CAB3274) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 120s.
