The Phospho SMO (S611/615) Rabbit Polyclonal Antibody (CABP0939) is a valuable tool for researchers studying the Hedgehog signaling pathway. Sonic Hedgehog (SHH) signaling plays a crucial role in embryonic development and tissue regeneration, as well as in various cancers and neurological disorders.This antibody specifically recognizes the phosphorylated form of the Smoothened (SMO) protein at serine 611 and 615 residues. By targeting these phosphorylation sites, researchers can investigate the activation and regulation of SMO in response to SHH signaling.
The antibody is suitable for use in Western blotting applications and has been validated for its specificity and sensitivity in detecting phospho-SMO in human samples.Understanding the phosphorylation of SMO is essential for unraveling the complex mechanisms of Hedgehog signaling and its implications in disease. This antibody provides a reliable tool for investigating the role of phospho-SMO in various cellular processes, making it a valuable resource for studies in cancer biology, developmental biology, and neurobiology.
Product Name:
Phospho-SMO-S611/615 Rabbit Polyclonal Antibody
SKU:
CABP0939
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S611 & S615 of human SMO (NP_005622.1).
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
Purification Method:
Affinity purification
Gene ID:
6608
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-SMO-S611/615 Rabbit pAb (CABP0939) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Jurkat cells were treated by Anisomycin (25 μg/mL) at 37℃ for 20 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
