The Phospho-Smo (S611/615/616) Rabbit Polyclonal Antibody (CABP0940) is a valuable tool for researchers studying the Sonic hedgehog (Shh) signaling pathway. This antibody targets the phosphorylated forms of Smoothened (Smo) protein, a key component of the Shh pathway, and is ideal for use in Western blot applications.Smo is a G protein-coupled receptor that plays a crucial role in transducing the Shh signal and regulating cell proliferation, differentiation, and survival during development. Phosphorylation of specific residues on Smo, such as S611, S615, and S616, can modulate its activity and downstream signaling, making it an important target for investigation in cancer and developmental biology research.
By using the Phospho-Smo (S611/615/616) Rabbit Polyclonal Antibody, researchers can detect and analyze the phosphorylation status of Smo in various cell types and tissues, providing valuable insights into the mechanisms underlying Shh signaling. This antibody is highly reactive with human samples and offers reliable and specific detection of phosphorylated Smo, making it a valuable tool for studies aimed at understanding the role of the Shh pathway in disease and development.
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
Purification Method:
Affinity purification
Gene ID:
6608
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-SMO-S611/615/616 Rabbit pAb (CABP0940) at 1:1000 dilution.293T-SMO cells were treated by SHH (0.4μg/mL) at 37℃ after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 1min.
