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Phospho-SMO-S611/615/616 Rabbit Polyclonal Antibody (CABP0940)

SKU:
CABP0940
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-SMO-S611/615/616 Rabbit Polyclonal Antibody
SKU:CABP0940
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S611 & S615 & S616 of human SMO (NP_005622.1).
Sequence:SADV SS
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:Gx; CRJS; PHLS; SMOH; FZD11; Phospho-SMO-S611/615/616
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Cell projection, Membrane, Multi-pass membrane protein, cilium.
Calculated MW:86kDa
Observed MW:80kDa

The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.

Purification Method:Affinity purification
Gene ID:6608
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from 293T cells, using Phospho-SMO-S611/615/616 Rabbit pAb (CABP0940) at 1:1000 dilution.293T-SMO cells were treated by SHH (0.4μg/mL) at 37℃ after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 1min.

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