The Phospho-SMC1A (S957) Polyclonal Antibody (CABP0090) is designed for research involving the phosphorylation of SMC1A at serine 957, a critical regulatory event in the activation of the SMC1A protein. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. It binds specifically to the phosphorylated form of SMC1A at serine 957, allowing for precise detection and quantification in various cell types.SMC1A is a key component of the cohesion complex, essential for sister chromatid cohesion and proper chromosome segregation during cell division. Phosphorylation of SMC1A at serine 957 is known to be involved in the regulation of DNA repair processes and cell cycle progression.
Research into the phosphorylation status of SMC1A at serine 957 can provide valuable insights into mechanisms of genome stability and cell proliferation.The Phospho-SMC1A (S957) Polyclonal Antibody (CABP0090) is a valuable tool for studying the role of SMC1A phosphorylation in various biological processes, including DNA damage response, cell cycle regulation, and genomic stability. Its high specificity and sensitivity make it an essential reagent for researchers in the fields of cell biology, cancer research, and molecular biology. Understanding the regulation of SMC1A phosphorylation at serine
Product Name:
Phospho-SMC1-S957 Rabbit Polyclonal Antibody
SKU:
CABP0090
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S957 of human SMC1A (NP_006297.2).
Sequence:
GSSQ G
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
8243
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-SMC1-S957 Rabbit pAb (CABP0090) at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by UV for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
