The Phospho-Smad5 (S465) Monoclonal Antibody is a valuable tool for studying the phosphorylation of Smad5 at serine 465, a key event in the TGF-beta signaling pathway. This antibody, produced using high quality monoclonal antibodies, specifically detects the phosphorylated form of Smad5 in human samples, making it an ideal choice for researchers studying signaling pathways involved in cell growth, differentiation, and development.The phosphorylation of Smad5 at serine 465 is known to play a crucial role in regulating the transcriptional activity of Smad proteins in response to TGF-beta signaling. By detecting and analyzing the phosphorylated form of Smad5, researchers can gain valuable insights into the regulatory mechanisms that control cell behavior and contribute to the development of diseases such as cancer, fibrosis, and developmental disorders.
This monoclonal antibody is validated for use in Western blot applications, allowing for accurate and reliable detection of phospho-Smad5 (S465) in various cell types and tissue samples. Its high specificity and sensitivity make it a valuable tool for studying the TGF-beta signaling pathway and its implications in physiological and pathological processes.
Product Name:
Phospho-Smad5-S465 Monoclonal Antibody
SKU:
CABP1411
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S465 of human Smad5.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:1000 - 1:5000
Synonyms:
DWFC; JV5-1; MADH5; Phospho-Smad5-S465
Positive Sample:
NIH/3T3,C6
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
52kDa
Observed MW:
58kDa
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4090
Clone Number:
ARC59204
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of NIH/3T3, using Phospho-Smad5-S465 Rabbit mAb (CABP1411) at 1:2000 dilution.NIH/3T3 cells were treated by BMP4(50ng/ml) at 37℃ for 6 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
