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Phospho-Smad5-S463/S465 Rabbit Monoclonal Antibody (CABP1023)

SKU:
CABP1023
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Smad5-S463/S465 Rabbit Monoclonal Antibody
SKU:CABP1023
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S463 & S465 of human Smad5 (Q99717).
Sequence:SSVS
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:DWFC; JV5-1; MADH5; Phospho-Smad5-S463/S465
Positive Sample:HeLa, Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus
Calculated MW:52kDa
Observed MW:52kDa

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:4090
Clone Number:ARC1568
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of HeLa cells, using Phospho-Smad5-S463/S465 antibody (CABP1023) at 1:1000 dilution or Smad5 antibody (CAB1947).Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

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