The Phospho-Smad5 (S463/S465) Rabbit Monoclonal Antibody (CABP1023) is a cutting-edge tool for research involving Smad5, a critical signaling molecule in the TGF-beta signaling pathway. This antibody, meticulously developed using rabbit monoclonal technology, offers high specificity and sensitivity when detecting phosphorylated Smad5 at serine residues 463 and 465 in human samples.Through its ability to bind specifically to phosphorylated Smad5, this antibody enables precise detection and quantification of Smad5 activation in various cell types and tissues. The phosphorylation of Smad5 at S463/S465 is known to play a crucial role in regulating cell growth, differentiation, and apoptosis, making this antibody an invaluable tool for researchers studying cellular signaling pathways, developmental biology, and cancer biology.
With its proven efficacy in Western blot applications, the Phospho-Smad5 (S463/S465) Rabbit Monoclonal Antibody (CABP1023) is a reliable choice for scientists conducting research in the fields of molecular biology, cell biology, and cancer research. By offering insights into the intricate mechanisms of TGF-beta signaling through the detection of phosphorylated Smad5, this antibody opens up new avenues for understanding and potentially targeting key pathways involved in disease progression and therapeutic intervention.
A synthetic phosphorylated peptide around S463 & S465 of human Smad5 (Q99717).
Sequence:
SSVS
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
DWFC; JV5-1; MADH5; Phospho-Smad5-S463/S465
Positive Sample:
HeLa, Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus
Calculated MW:
52kDa
Observed MW:
52kDa
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4090
Clone Number:
ARC1568
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-Smad5-S463/S465 antibody (CABP1023) at 1:1000 dilution or Smad5 antibody (CAB1947).Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
