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Phospho-Smad5-S463/S465 Polyclonal Antibody (CABP1319)

SKU:
CABP1319
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IHC
€139 - €489
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Smad5-S463/S465 Polyclonal Antibody
SKU:CABP1319
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S463 & S465 of human Phospho-Smad5-S463/S465 (NP_005894.3).
Sequence:SSVS
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:DWFC; JV5-1; MADH5; Phospho-Smad5-S463/S465
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, nucleoplasm, nucleus
Calculated MW:52kDa

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:4090
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Phospho-Smad5-S463/S465 Rabbit pAb (CABP1319) at 1:600 dilution.NIH/3T3 cells were treated by BMP4 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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