The Phospho-Smad5 (S463/S465) Polyclonal Antibody offered by AssayGenie is a valuable tool for researchers studying the Smad5 protein, a key player in the TGF-beta signaling pathway. This antibody, generated in rabbits, specifically recognizes the phosphorylated form of Smad5 at serine residues 463 and 465. Validated for use in Western blot applications, this antibody allows for the detection and analysis of phosphorylated Smad5 in various cell types.The TGF-beta signaling pathway is essential for regulating cell growth, differentiation, and development, making the study of phosphorylated Smad5 crucial for understanding this important biological process.
Dysregulation of the TGF-beta pathway has been implicated in a variety of diseases, including cancer, fibrosis, and immune disorders. By targeting phosphorylated Smad5, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the Phospho-Smad5 (S463/S465) Polyclonal Antibody from AssayGenie is a valuable research tool for investigating the TGF-beta signaling pathway and its role in disease. Its high reactivity with human samples and specificity for phosphorylated Smad5 make it an ideal choice for studies in molecular biology, cancer research, and beyond.
Product Name:
Phospho-Smad5-S463/S465 Polyclonal Antibody
SKU:
CABP1319
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S463 & S465 of human Phospho-Smad5-S463/S465 (NP_005894.3).
Sequence:
SSVS
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:
DWFC; JV5-1; MADH5; Phospho-Smad5-S463/S465
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol, nucleoplasm, nucleus
Calculated MW:
52kDa
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4090
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Smad5-S463/S465 Rabbit pAb (CABP1319) at 1:600 dilution.NIH/3T3 cells were treated by BMP4 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
