The SMAD5 Polyclonal Antibody (CAB0635) is a valuable tool for research involving SMAD5, a crucial protein in the TGF-β signaling pathway. This antibody, generated in rabbits, is highly specific for SMAD5 and has been validated for use in Western blot applications.SMAD5 is a transcription factor that plays a key role in mediating the biological effects of TGF-β signaling, regulating crucial cellular processes such as cell proliferation, differentiation, and apoptosis.
Dysregulation of SMAD5 signaling has been implicated in various diseases, including cancer, cardiovascular disorders, and developmental abnormalities.With its high specificity and sensitivity, the SMAD5 Polyclonal Antibody enables precise detection and quantification of SMAD5 levels in a variety of sample types, making it an invaluable tool for researchers studying the TGF-β signaling pathway and its implications in disease development and progression.
Product Name:
Smad5 Rabbit Polyclonal Antibody
SKU:
CAB0635
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 140-240 of human Smad5 (NP_005894.3).
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4090
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat lung, using Smad5 Rabbit pAb (CAB0635) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
