The Phospho-Smad3 (T179) Polyclonal Antibody (CABP0554) is a vital tool for researchers studying the phosphorylation of Smad3 at threonine 179, which is involved in the TGF-beta signaling pathway. This antibody, produced in rabbits, has high specificity for human samples and is validated for Western blot applications. It specifically binds to the phosphorylated form of Smad3 at threonine 179, enabling precise detection and analysis in a variety of cell types.The TGF-beta signaling pathway plays a crucial role in regulating cell growth, differentiation, and development, as well as in cancer progression and fibrosis. Phosphorylation of Smad3 at threonine 179 is a key event in this pathway, affecting downstream gene expression and cellular responses.
Studying this phosphorylation event is essential for understanding the intricate mechanisms of TGF-beta signaling and its implications in various diseases.Researchers in the fields of cell biology, cancer biology, and signaling pathways will find the Phospho-Smad3 (T179) Polyclonal Antibody to be a valuable tool for their studies. Its high specificity and reliability make it an ideal choice for investigating the role of phosphorylated Smad3 in cellular processes and disease development.
Product Name:
Phospho-Smad3-T179 Rabbit Polyclonal Antibody
SKU:
CABP0554
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T179 of human Smad3 (NP_005893.1).
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.
Purification Method:
Affinity purification
Gene ID:
4088
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-Smad3-T179 Rabbit pAb (CABP0554). The lane on the right is treated with the antigen-specific peptide.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
