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Phospho-Smad3-S423/S425 Rabbit Monoclonal Antibody (CABP0727)

SKU:
CABP0727
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Immunology
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Smad3-S423/S425 Rabbit Monoclonal Antibody
SKU:CABP0727
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S423 & S425 of human Smad3 (P84022).
Sequence:SSVS
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200
Synonyms:LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436; Phospho-Smad3-S423/S425
Positive Sample:C6+PMA/TPA
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:48kDa
Observed MW:55kDa

The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.

Purification Method:Affinity purification
Gene ID:4088
Clone Number:ARC0170
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C6 cells, using Phospho-Smad3-S423/S425 Rabbit mAb (CABP0727) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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