The Phospho-Smad3 (S423/S425) Rabbit Monoclonal Antibody (CABP0727) is a high-quality antibody specifically designed for research involving the phosphorylation of Smad3 at serine residues 423 and 425. Smad3 is a critical protein involved in the TGF-β signaling pathway, playing a significant role in cell growth, differentiation, and apoptosis.This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By specifically targeting the phosphorylated form of Smad3, researchers can effectively study the activation and regulation of the TGF-β signaling pathway in various cell types.
This makes the CABP0727 antibody ideal for studies in cell signaling, developmental biology, and cancer research.The phosphorylation of Smad3 at serine residues 423 and 425 is known to regulate its transcriptional activity, affecting downstream gene expression and cellular responses to TGF-β signaling. Understanding the phosphorylation of Smad3 is crucial for elucidating its role in disease processes such as cancer, fibrosis, and immune disorders. The CABP0727 antibody provides researchers with a reliable tool for investigating the intricate mechanisms of Smad3 phosphorylation and its implications in health and disease.
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.
Purification Method:
Affinity purification
Gene ID:
4088
Clone Number:
ARC0170
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-Smad3-S423/S425 Rabbit mAb (CABP0727) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
