The Phospho-SMAD2 (T220) Rabbit Polyclonal Antibody (CABP0909) is a valuable tool for researchers studying the phosphorylation of SMAD2, a key signaling molecule in the TGF-beta pathway. This antibody, raised in rabbits, is highly specific for the phosphorylated form of SMAD2 at threonine 220 and is validated for use in Western blot applications. By targeting the phosphorylated form of SMAD2, researchers can track the activation of the TGF-beta pathway in various cell types and investigate its role in diseases such as cancer, fibrosis, and immune disorders.SMAD2 is a critical mediator of TGF-beta signaling, playing a central role in regulating cell growth, differentiation, and apoptosis.
Phosphorylation of SMAD2 at threonine 220 is known to enhance its transcriptional activity and influence downstream gene expression patterns. By using the Phospho-SMAD2 (T220) Rabbit Polyclonal Antibody, researchers can gain valuable insights into the molecular mechanisms underlying TGF-beta signaling and its impact on cellular processes. This antibody is an essential tool for studies aiming to elucidate the role of SMAD2 phosphorylation in health and disease.
Product Name:
Phospho-Smad2-T220 Rabbit Polyclonal Antibody
SKU:
CABP0909
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T220 of human Smad2 (NP_005892.1).
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
4087
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using Phospho-Smad2-T220 Rabbit pAb (CABP0909) at 1:500 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
