The Phospho-SMAD2 (S467) Polyclonal Antibody (CABP0269) is a valuable tool for researchers studying the SMAD2 signaling pathway. SMAD2 is a key player in the TGF-beta signaling pathway, regulating processes such as cell growth, differentiation, and apoptosis. This antibody, produced in rabbits, shows high specificity for phosphorylated SMAD2 at the S467 site in human samples.Validation studies have shown that this antibody is suitable for use in Western blot applications, allowing for the detection and analysis of phosphorylated SMAD2 in various cell types. By targeting the specific phosphorylation site, researchers can gain insights into the activation and regulation of the SMAD2 pathway, providing valuable information for studies in cell biology, cancer research, and developmental biology.
Understanding the phosphorylation status of SMAD2 at the S467 site is crucial for unraveling the intricate signaling mechanisms involved in TGF-beta signaling. Research using this antibody may lead to new insights into how dysregulation of the SMAD2 pathway contributes to disease development, paving the way for the development of targeted therapies for conditions such as cancer and fibrosis.
Product Name:
Phospho-Smad2-S467 Rabbit Polyclonal Antibody
SKU:
CABP0269
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S467 of human Smad2 (NP_001003652.1).
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
4087
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-Smad2-S467 Rabbit pAb (CABP0269) at 1:1000 dilution.Hela cells were treated by TGF-β (10 ng/ml) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
