The Phospho-Smad2 (S465/S467)/Smad3 (S423/S425) Monoclonal Antibody is a valuable tool for researchers studying the Smad signaling pathway, which plays a critical role in cell growth, differentiation, and development. This antibody, generated using advanced monoclonal antibody technology, specifically targets phosphorylated Smad2 at serine residues 465 and 467, as well as phosphorylated Smad3 at serine residues 423 and 425.One of the key functions of the Smad signaling pathway is its involvement in TGF-β signaling, a crucial pathway in regulating cell proliferation, differentiation, and apoptosis. By targeting phosphorylated Smad2 and Smad3, this antibody enables the detection and analysis of active Smad proteins in various cell types and tissues, providing valuable insights into their role in cellular processes.
This highly specific and sensitive antibody is suitable for Western blot applications and is validated for use with human samples, making it an ideal tool for researchers studying signal transduction pathways, cell biology, and cancer research. By understanding the phosphorylation status of Smad2 and Smad3, researchers can gain valuable insights into the mechanisms underlying various diseases, including cancer, fibrosis, and developmental disorders.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
Purification Method:
Affinity purification
Gene ID:
4087/4088
Clone Number:
ARC56314
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb (CABP1343) at1:2000 dilution.HeLa and NIH/3T3 cells were treated by TGF-β3（H） (200 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
