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Phospho-Smad2-S465/S467 + Smad3-S423/S425 Monoclonal Antibody (CABP1343)

SKU:
CABP1343
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $731
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Smad2-S465/S467 + Smad3-S423/S425 Monoclonal Antibody
SKU:CABP1343
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S465 & S467 of human SMAD2 (NP_005892.1).
Sequence:SSMS
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; Phospho-Smad2-S465/S467 + Smad3-S423/S425
Positive Sample:HeLa TGF-β3,NIH/3T3 TGF-β3,C6 TGF-β3
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:48kDa/52kDa/25kDa/35kDa/43kDa
Observed MW:52kDa/60kDa

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Purification Method:Affinity purification
Gene ID:4087/4088
Clone Number:ARC56314
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb (CABP1343) at1:2000 dilution.HeLa and NIH/3T3 cells were treated by TGF-β3（H） (200 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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