The Phospho-Smad2 (S465/S467) Rabbit Polyclonal Antibody (CABP0925) is a valuable tool for researchers studying the Smad2 protein, a key regulator in the TGF-β signaling pathway. This antibody is raised in rabbits and is highly specific to the phosphorylated form of Smad2 at serine residues 465 and 467 in human samples. Validated for use in Western blot applications, this antibody allows for the detection and analysis of phospho-Smad2 in various cell types.Smad2 is a crucial mediator of TGF-β signaling, playing a pivotal role in cell growth, differentiation, and apoptosis. Phosphorylation at serine residues 465 and 467 is known to regulate Smad2 activity, making this antibody an essential tool for researchers investigating the TGF-β signaling pathway in immunology, cancer research, and developmental biology.
By targeting phospho-Smad2, researchers can gain insights into the molecular mechanisms underlying TGF-β signaling and its impact on various cellular processes. This antibody's high specificity and sensitivity make it an ideal choice for studies aimed at unraveling the complex interplay between Smad2 phosphorylation and cellular responses, paving the way for potential therapeutic interventions targeting the TGF-β pathway.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
4087
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-Smad2-S465/S467 Rabbit pAb (CABP0925) at 1:1000 dilution.HeLa cells were treated by TGF-β (10ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
