The Phospho-Smad2 (S250) Rabbit Monoclonal Antibody (CABP1007) is a research tool designed for studying the phosphorylation of the Smad2 protein, a key player in the TGF-β signaling pathway. This antibody, produced in rabbits, is highly specific for phosphorylated Smad2 at the S250 site in human samples, making it an excellent choice for Western blot applications.Smad2 is a crucial mediator of TGF-β signaling, regulating various cellular processes such as cell proliferation, differentiation, and apoptosis. Phosphorylation of Smad2 at the S250 site is known to be important for its activation and subsequent translocation into the nucleus to regulate gene expression.
By using the Phospho-Smad2 (S250) Rabbit Monoclonal Antibody, researchers can investigate the phosphorylation status of Smad2 in different cell types and conditions, providing valuable insights into the role of TGF-β signaling in processes such as development, tissue homeostasis, and disease progression. This antibody is a valuable tool for studies in cell biology, cancer research, and other fields where TGF-β signaling is of interest.
Product Name:
Phospho-Smad2-S250 Rabbit Monoclonal Antibody
SKU:
CABP1007
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S250 of human Smad2 (Q15796).
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
4087
Clone Number:
ARC1552
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-Smad2-S250 Rabbit mAb (CABP1007) at 1:1000 dilution.293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.C2C12 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
