The Phospho-SMAD1 (S463/465) / SMAD5 (S463/465) / SMAD9 (S465/467) Polyclonal Antibody (CABP0850) is a valuable tool for researchers studying the SMAD signaling pathway in various biological processes. This antibody, produced in rabbits, is highly specific to phosphorylated forms of SMAD1, SMAD5, and SMAD9, allowing for precise detection and analysis in Western blot experiments.SMAD proteins are key mediators of the TGF-β signaling pathway, playing crucial roles in cell growth, differentiation, and development. Phosphorylation of SMAD proteins is known to regulate their activity, influencing downstream gene expression and cellular responses.
The Phospho-SMAD1/5/9 Polyclonal Antibody enables researchers to study the phosphorylation status of these important signaling molecules, providing insights into their functional roles in various cellular processes.By targeting phosphorylated SMAD1, SMAD5, and SMAD9, this antibody is particularly useful for investigating signaling events in cancer, fibrosis, and other diseases where TGF-β pathway dysregulation is implicated. Its high specificity and sensitivity make it a valuable tool for understanding the complex mechanisms of SMAD signaling and its impact on cellular physiology and disease pathology.
cytoplasm, cytosol, homomeric SMAD protein complex, nucleoplasm, nucleus
Calculated MW:
60kDa
Observed MW:
60kDa
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
Purification Method:
Affinity purification
Gene ID:
4086/4090/4093
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-Smad1(Ser463/465)/Smad5(Ser463/465)/Smad9(Ser465/467) pAb (CABP0850) at 1:1000 dilution.HeLa cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
