The Phospho SF3B1 (T313) Polyclonal Antibody (CABP0844) is a valuable tool for researchers studying SF3B1, a key protein involved in RNA splicing and gene expression regulation. This antibody, raised in rabbits, is highly specific to the phosphorylated form of SF3B1 at threonine 313 and is validated for use in Western blot applications. It enables detection and analysis of phosphorylated SF3B1 in various cell types, providing insight into its role in cellular processes such as pre-mRNA splicing.SF3B1 is a critical component of the spliceosome, a complex machinery responsible for removing introns from pre-mRNA molecules. Mutations in SF3B1 have been associated with various types of cancer, making it an attractive target for therapeutic development.
The phosphorylation of SF3B1 at threonine 313 has been implicated in regulating its activity and function, highlighting the importance of studying this post-translational modification in cancer and other diseases.By using the Phospho SF3B1 (T313) Polyclonal Antibody, researchers can gain a better understanding of the role of SF3B1 phosphorylation in RNA splicing and its potential implications for disease pathology. This antibody is an essential tool for investigations into the molecular mechanisms underlying gene expression dysregulation in cancer and other disorders, ultimately leading to the development of novel therapeutic strategies targeting SF3B1.
Product Name:
Phospho-SF3B1-T313 Rabbit Polyclonal Antibody
SKU:
CABP0844
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T313 of human SF3B1 (NP_036565.2).
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
23451
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-SF3B1-T313 Rabbit pAb (CABP0844) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
