The Phospho-RPS6KA5 (S376) Polyclonal Antibody (CABP0800) is a powerful tool for researchers investigating the phosphorylation of RPS6KA5, a protein kinase involved in cellular signaling pathways. This antibody, produced in rabbits, has been optimized for detection of phosphorylation at serine 376 in human samples, making it a valuable asset for studies on cell signaling, cancer biology, and drug development.RPS6KA5, also known as ribosomal protein S6 kinase A5, is a key player in regulating cell growth, proliferation, and differentiation through its phosphorylation of target proteins. Phosphorylation at serine 376 has been linked to specific cellular responses and may serve as a biomarker for various diseases and conditions.
The Phospho-RPS6KA5 (S376) Polyclonal Antibody enables precise detection and quantification of phosphorylated RPS6KA5, allowing researchers to explore its role in cellular processes and disease pathology.By using this antibody in techniques such as Western blotting, researchers can gain insights into the activation status and functional implications of RPS6KA5 phosphorylation. With its high specificity and sensitivity, this antibody is a valuable tool for advancing our understanding of cellular signaling mechanisms and potential therapeutic targets in various pathological conditions.
Product Name:
Phospho-RPS6KA5-S376 Rabbit Polyclonal Antibody
SKU:
CABP0800
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S376 of human RPS6KA5 (NP_004746.2).
Sequence:
GYSF V
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:100
Synonyms:
MSK1; RLPK; MSPK1; Phospho-RPS6KA5-S376
Positive Sample:
NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
90kDa
Observed MW:
90kDa
Enables ATP binding activity and protein serine/threonine kinase activity. Involved in several processes, including histone-serine phosphorylation; positive regulation of histone modification; and regulation of transcription, DNA-templated. Located in cytoplasm and nucleoplasm.
Purification Method:
Affinity purification
Gene ID:
9252
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RPS6KA5-S376 Rabbit pAb (CABP0800) at 1:2000 dilution. NIH/3T3 cells were treated by Anisomycin (25μg/mL) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
