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Phospho-RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 (Ser221/227/S218/232) Antibody (PACO24121)

SKU:
PACO24121
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
$659
Frequently bought together:

Description

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Phospho-RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 (Ser221/227/S218/232) Antibody (PACO24121)

The Phospho-RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 (Ser221/227, S218/232) Antibody (PAC024121) is a highly specific and sensitive tool for studying the phosphorylation of RPS6KA1, RPS6KA3, RPS6KA2, and RPS6KA6 proteins. This polyclonal antibody, produced in rabbits, is validated for use in various applications including Western blot and immunohistochemistry. It binds to the phosphorylated forms of these proteins at specific serine residues, allowing for the detection and analysis of their activation status in cellular signaling pathways.The RPS6KA family of proteins plays a crucial role in translating extracellular signals into cellular responses, regulating processes such as cell growth, differentiation, and survival.

Phosphorylation of RPS6KA1, RPS6KA3, RPS6KA2, and RPS6KA6 at serine residues 221/227 and 218/232 is known to activate their kinase activity, leading to downstream signaling cascades that impact gene expression and cell function. Studying the phosphorylation status of these proteins can provide valuable insights into the regulation of cellular processes and pathophysiological states associated with cancer, metabolic disorders, and neurodegenerative diseases.By using the Phospho-RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6

Antibody Name:Phospho-RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 (Ser221/227/S218/232) Antibody (PACO24121)
Antibody SKU:PACO24121
Size:100ul
Host Species:Rabbit
Tested Applications:ELISA, WB
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:Human, Mouse
Immunogen:Peptide sequence around phosphorylation site of Serine 221/227/218/232(A-Y-S(p)-F-C) derived from Human RSK1/2/3/4.
Form:Liquid
Storage Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern blot analysis of extracts from HepG2 cells (Lane 2), using RSK1/2/3/4 (Phospho-Ser221/227/218/232) Antibody. The lane on the left is treated with antigen-specific peptide.
Background:

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway.

Synonyms:KS6A1; KS6AA; MAPKAP-K1a; RSK1;
UniProt Protein Function:p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.
UniProt Protein Details:

Protein type:EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Translation; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: 1p

Cellular Component: nucleoplasm; cytoplasm; spindle; cytosol; nucleus

Molecular Function:protein serine/threonine kinase activity; protein binding; caspase inhibitor activity; protein serine/threonine/tyrosine kinase activity; magnesium ion binding; ATP binding

Biological Process: regulation of transcription in response to stress; axon guidance; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; regulation of translation in response to stress; toll-like receptor 3 signaling pathway; negative regulation of caspase activity; cell cycle; signal transduction; protein amino acid phosphorylation; positive regulation of cell growth; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; synaptic transmission; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; positive regulation of cell differentiation; toll-like receptor 4 signaling pathway; negative regulation of apoptosis

NCBI Summary:This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:Q15418
NCBI GenInfo Identifier:20178306
NCBI Gene ID:6195
NCBI Accession:Q15418.2
UniProt Secondary Accession:Q15418,Q5SVM5, Q5SVM8, Q5SVM9, Q96C05, Q9BQK2, A6NGG4 A8K9K7, B2RDY8, B7Z5J0,
UniProt Related Accession:Q15418
Molecular Weight:72,698 Da
NCBI Full Name:Ribosomal protein S6 kinase alpha-1
NCBI Synonym Full Names:ribosomal protein S6 kinase, 90kDa, polypeptide 1
NCBI Official Symbol:RPS6KA1  
NCBI Official Synonym Symbols:RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A  
NCBI Protein Information:ribosomal protein S6 kinase alpha-1; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1; S6K-alpha 1; S6K-alpha-1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); p90-RSK 1; p90RSK1; p90S6K; ribosomal S6 kinase 1; ribosomal protein S6 kinase alpha 1
UniProt Protein Name:Ribosomal protein S6 kinase alpha-1
UniProt Synonym Protein Names:90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1; RSK-1
Protein Family:Ribosomal protein S6 kinase
UniProt Gene Name:RPS6KA1  
UniProt Entry Name:KS6A1_HUMAN
AntibodiesELISA Kits
Phospho-RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 (S221/227/S218/232) Antibody (PACO04956)RSK1/2/3/4 Colorimetric Cell-Based ELISA Kit
RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 Antibody (PACO04957)RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Colorimetric Cell-Based ELISA Kit
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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