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Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit Polyclonal Antibody (CABP0537)

SKU:
CABP0537
Product Type:
Antibody
Applications:
WB
IP
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 16 Anti-Phospho-RPS6-S240/244 Antibody CABP0537
  • Cell Biology Antibodies 16 Anti-Phospho-RPS6-S240/244 Antibody CABP0537
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Description

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Product Name:Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit Polyclonal Antibody
SKU:CABP0537
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S240 & S244 of human RPS6 (NP_001001.2).
Sequence:STSK S
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:S6; eS6; Phospho-S6 Ribosomal Protein (RPS6)-S240/244
Positive Sample:HeLa,NIH/3T3
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytoplasmic ribonucleoprotein granule, cytosol, cytosolic ribosome, endoplasmic reticulum, nucleolus, nucleoplasm, nucleus, perinuclear region of cytoplasm
Calculated MW:29kDa
Observed MW:36kDa

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Purification Method:Affinity purification
Gene ID:6194
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit pAb (CABP0537) at 1:1000 dilution or RPS6 antibody (CAB6058). HeLa cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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