Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit Polyclonal Antibody (CABP0537)
The Phospho-RPS6 (S240-244) Polyclonal Antibody (CABP0537) is a powerful tool for researchers studying the phosphorylation of ribosomal protein S6 (RPS6) at residues Ser240-244. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to the phosphorylated RPS6 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in cell signaling and protein phosphorylation.The phosphorylation of RPS6 at Ser240-244 is a critical regulatory event in the mTOR signaling pathway, which plays a key role in cell growth, proliferation, and metabolism.
Dysregulation of this pathway has been implicated in a variety of diseases, including cancer, diabetes, and neurological disorders. By studying the phosphorylation status of RPS6, researchers can gain valuable insights into the mechanisms underlying these diseases and discover potential targets for therapeutic intervention.Overall, the Phospho-RPS6 (S240-244) Polyclonal Antibody is a valuable resource for researchers investigating the mTOR signaling pathway and its implications in health and disease. Its high specificity and sensitivity make it an indispensable tool for studies in cell biology, oncology, and metabolic disorders.
Product Name:
Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit Polyclonal Antibody
SKU:
CABP0537
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S240 & S244 of human RPS6 (NP_001001.2).
Sequence:
STSK S
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
S6; eS6; Phospho-S6 Ribosomal Protein (RPS6)-S240/244
Positive Sample:
HeLa,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytoplasmic ribonucleoprotein granule, cytosol, cytosolic ribosome, endoplasmic reticulum, nucleolus, nucleoplasm, nucleus, perinuclear region of cytoplasm
Calculated MW:
29kDa
Observed MW:
36kDa
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Purification Method:
Affinity purification
Gene ID:
6194
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit pAb (CABP0537) at 1:1000 dilution or RPS6 antibody (CAB6058). HeLa cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
