Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit Polyclonal Antibody (CABP0538)
The Phospho-RPS6 (S235/236) Polyclonal Antibody (CABP0538) is a valuable tool for researchers investigating the phosphorylation status of RPS6 at serine 235 and 236 residues. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it suitable for use in Western blot applications.RPS6 is a ribosomal protein that plays a crucial role in translational regulation and cell growth. Phosphorylation of RPS6 at serine 235 and 236 is known to be important for its function in controlling protein synthesis. This antibody specifically binds to the phosphorylated form of RPS6, allowing for the detection and analysis of phosphorylation events in various cell types.
Research into the phosphorylation of RPS6 is essential for understanding its role in cell signaling pathways, growth regulation, and disease processes. The Phospho-RPS6 (S235/236) Polyclonal Antibody provides a valuable tool for studying these mechanisms and may have applications in cancer research, cell biology, and drug development efforts aimed at targeting RPS6 activity.
Product Name:
Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit Polyclonal Antibody
SKU:
CABP0538
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S235 & S236 of human RPS6 (NP_001001.2).
Sequence:
RLSS LR
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
S6; eS6; Phospho-S6 Ribosomal Protein (RPS6)-S235/236
Positive Sample:
HeLa,NIH/3T3,PC-12
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytoplasmic ribonucleoprotein granule, cytosol, cytosolic ribosome, endoplasmic reticulum, nucleolus, nucleoplasm, nucleus, perinuclear region of cytoplasm
Calculated MW:
29kDa
Observed MW:
32kDa
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Purification Method:
Affinity purification
Gene ID:
6194
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-S6 Ribosomal Protein (RPS6)-S235/236 antibody (CABP0538) at 1:1000 dilution.HeLa cells NIH/3T3 cells and PC-12 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
