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Anti-Phospho-RPA2-S4/S8 Antibody (CABP1102)

SKU:
CABP1102
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
€129 - €489
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Description

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Anti-Phospho-RPA2-S4/S8 Antibody (CABP1102)

The Phospho-RPA2 (S4/S8) Rabbit Polyclonal Antibody (CABP1102) is specifically designed for researchers studying the phosphorylation of RPA2 at serine 4 and serine 8. RPA2 is a key protein in DNA repair and replication processes, and its phosphorylation at these sites is essential for cell cycle progression and genome stability. This high-quality antibody, raised in rabbits, offers high reactivity with human samples and has been validated for use in Western blot applications. By binding to the phosphorylated forms of RPA2, this antibody allows for the precise detection and analysis of RPA2 phosphorylation in various cell types.

Given the crucial role of RPA2 phosphorylation in maintaining genomic integrity, this antibody is ideal for research in the fields of DNA damage response, cancer biology, and cell cycle regulation. Understanding the mechanisms and consequences of RPA2 phosphorylation at serine 4 and serine 8 can provide valuable insights into disease processes and potential therapeutic targets.

Antibody Name:Anti-Phospho-RPA2-S4/S8 Antibody
Antibody SKU:CABP1102
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A phospho specific peptide corresponding to residues surrounding S4/S8 of human RPA2.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:
Immunogen:A phospho specific peptide corresponding to residues surrounding S4/S8 of human RPA2.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:6118
Uniprot:P15927
Cellular Location:Nucleus, PML body
Calculated MW:29kDa/30kDa/38kDa
Observed MW:Refer to figures
Synonyms:REPA2, RP-A p32, RP-A p34, RPA32, RPA2, RP-Ap32, RP-Ap34
Background:As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
UniProt Protein Function:RFA2: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA replication

Chromosomal Location of Human Ortholog: 1p35

Cellular Component: chromatin; chromosome, telomeric region; DNA replication factor A complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body

Molecular Function:damaged DNA binding; enzyme binding; protein binding; protein N-terminus binding; protein phosphatase binding; single-stranded DNA binding; ubiquitin protein ligase binding

Biological Process: base-excision repair; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA repair; DNA replication; DNA strand elongation during DNA replication; double-strand break repair; double-strand break repair via homologous recombination; error-prone postreplication DNA repair; G1 DNA damage checkpoint; G1/S transition of mitotic cell cycle; mismatch repair; mitotic cell cycle; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; telomere maintenance; telomere maintenance via recombination; telomere maintenance via semi-conservative replication; transcription-coupled nucleotide-excision repair

UniProt Code:P15927
NCBI GenInfo Identifier:132474
NCBI Gene ID:6118
NCBI Accession:P15927.1
UniProt Secondary Accession:P15927,Q52II0, Q5TEI9, Q5TEJ5,
UniProt Related Accession:P15927
Molecular Weight:38,810 Da
NCBI Full Name:Replication protein A 32 kDa subunit
NCBI Synonym Full Names:replication protein A2
NCBI Official Symbol:RPA2  
NCBI Official Synonym Symbols:REPA2; RPA32; RP-A p32; RP-A p34  
NCBI Protein Information:replication protein A 32 kDa subunit
UniProt Protein Name:Replication protein A 32 kDa subunit
UniProt Synonym Protein Names:Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34
Protein Family:Replication protein
UniProt Gene Name:RPA2  
UniProt Entry Name:RFA2_HUMAN

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