The Phospho-RIPK1 (S166) Monoclonal Antibody is a powerful tool for researchers studying cell signaling pathways and inflammatory responses. This antibody specifically targets the phosphorylated form of RIPK1 at serine 166, a critical site for regulating RIPK1 activity in cell survival and death pathways.RIPK1, or Receptor-Interacting Protein Kinase 1, is a key player in the regulation of cell death and inflammation. Phosphorylation of RIPK1 at serine 166 has been shown to influence its function in various cellular processes, including necroptosis, apoptosis, and inflammation. By targeting this phosphorylation site, researchers can gain valuable insights into the role of RIPK1 in disease states such as cancer, neurodegenerative disorders, and inflammatory conditions.
This monoclonal antibody, produced through cutting-edge technology, is highly specific and sensitive, making it an ideal choice for detecting and quantifying phospho-RIPK1 (S166) in cell lysates and tissue samples. Its versatility in applications such as Western blotting, immunofluorescence, and immunoprecipitation allows for detailed analysis of RIPK1 signaling pathways in various experimental systems.Overall, the Phospho-RIPK1 (S166) Monoclonal Antibody offers researchers a reliable tool for investigating the complex role of RIPK1 in cell signaling and inflammatory responses, paving the way for potential therapeutic interventions targeting
Product Name:
Phospho-RIPK1-S166 Monoclonal Antibody
SKU:
CABP1448
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S166 of human RIPK1(NP_003795.2).
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth.
Purification Method:
Affinity purification
Gene ID:
8737
Clone Number:
ARC62873
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of HT-29 cells, using Phospho-RIPK1-S166 Rabbit mAb (CABP1448) at 1:4000 dilution.HT-29 cells were treated with Z-VAD (50uM), human TNF-α (50ng/ml) and BV-6 (200nM) at 37℃ for 5 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
