The Phospho-RelB (S573) Polyclonal Antibody is a valuable tool for researchers studying the phosphorylation of the RelB protein, an important transcription factor involved in regulating immune and inflammatory responses. This antibody, produced in rabbits, specifically binds to the phosphorylated form of RelB at serine 573, allowing for the detection and analysis of this post-translational modification in various cell types.RelB is a key player in the NF-κB signaling pathway, which is known to be dysregulated in various diseases including cancer, autoimmune disorders, and chronic inflammatory conditions. Understanding the phosphorylation status of RelB at S573 can provide insights into the regulation of NF-κB activity and its role in disease pathogenesis.
Validated for use in Western blot applications with human samples, this antibody is a valuable tool for researchers interested in studying the signaling pathways and molecular mechanisms that govern immune responses and inflammation. By targeting the phosphorylated form of RelB, researchers can gain a deeper understanding of how this transcription factor contributes to the development and progression of various diseases, ultimately paving the way for the development of novel therapeutic strategies.
Product Name:
Phospho-RELB-S573 Polyclonal Antibody
SKU:
CABP1297
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around S573 of human RELB (NP_006500.2).
Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity and protein kinase binding activity. Involved in lymphocyte differentiation and negative regulation of interferon-beta production. Located in cytosol and nucleoplasm. Part of chromatin; nucleus; and transcription repressor complex. Colocalizes with centrosome. Implicated in breast cancer and immunodeficiency 53. Biomarker of breast cancer and transitional cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
5971
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from A20, using Phospho-RELB-S573 Rabbit pAb (CABP1297) at 1:500 dilution.A20 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
