The Phospho-RelA (T435) Polyclonal Antibody (CABP0838) is a vital tool for researchers studying the phosphorylation of the RelA protein, a key regulator of the NF-κB signaling pathway. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the phosphorylated form of RelA at threonine 435, this antibody enables precise detection and analysis of this critical post-translational modification in various cell types.The NF-κB signaling pathway plays a crucial role in inflammation, immune response, and cell survival, making it a focal point in research on cancer, autoimmune disorders, and inflammatory diseases.
The phosphorylation of RelA at threonine 435 is known to modulate NF-κB activity, highlighting the importance of investigating this specific modification in understanding disease mechanisms and developing targeted therapies. Researchers can use this antibody to explore the impact of phosphorylated RelA on NF-κB signaling, advancing knowledge in the fields of immunology and cancer research.
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5970
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-kB p65/RelA-T434 pAb (CABP0838) at 1:1000 dilution or RelA antibody (CAB16271).NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5min.
