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Phospho-NF-kB p65/RelA-S536 Rabbit Polyclonal Antibody (CABP0475)

SKU:
CABP0475
Product Type:
Antibody
Applications:
WB
IHC
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • Immunology Antibodies 3 Anti-Phospho-RELA-S536 Antibody CABP0475
  • Immunology Antibodies 3 Anti-Phospho-RELA-S536 Antibody CABP0475
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-NF-kB p65/RelA-S536 Rabbit Polyclonal Antibody
SKU:CABP0475
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S536 of human NF-kB p65/RelA (NP_068810.3).
Sequence:FSSI A
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-S536
Positive Sample:HeLa+TNF-α,NIH/3T3+TNF-α,C6+TNF-α
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:60kDa
Observed MW:65kDa

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5970
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (CABP0475) at 1:1000 dilution. HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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