The Phospho-RelA (S536) Polyclonal Antibody (CABP0124) is a valuable tool for researchers studying the NF-κB pathway, a critical signaling pathway involved in inflammation, immunity, and cancer. This antibody, produced in rabbits, specifically targets the phosphorylated form of RelA at serine 536, allowing for precise detection and analysis in Western blot applications.RelA, also known as p65, is a key component of the NF-κB complex and plays a central role in the activation of target genes involved in immune and inflammatory responses. Phosphorylation of RelA at serine 536 is a critical regulatory event that enhances its transcriptional activity, making it a key target for studying NF-κB signaling dynamics.
The Phospho-RelA (S536) Polyclonal Antibody is highly specific for human samples and is validated for use in a variety of cell types, making it a versatile tool for immunology, cancer research, and drug discovery studies. By targeting the phosphorylated form of RelA, researchers can gain valuable insights into the regulation of NF-κB signaling and its impact on various disease states.
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5970
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (CABP0124) at 1:10000 dilution. HeLa cells were treated by TNF-α (50 ng/ml) and Calyculin A (50 nM) at 37℃ for 10 minutes.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 30s.
